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Available courses

Microbiology and Immunology

Course Summary: BBT302 – Microbiology and Immunology

Course Code: BBT302
Programme: B.Tech Biotechnology
Semester: 3rd Semester (Second Year)
Credits: 4 (3L + 1T)
Contact Hours: 4 hours/week

Course Objective:

To impart foundational and applied knowledge of microbiology and immunology, emphasizing microbial diversity, classification, physiology, pathogenicity, and host immune responses. The course integrates basic microbial techniques and immunological principles relevant to diagnostics and therapeutics.

Course Content Overview:

Unit I: Fundamentals of Microbiology

  • History, scope, and development of microbiology

  • Classification of microorganisms: Bacteria, Fungi, Algae, Protozoa, and Viruses

  • Microscopy: Principles of light and electron microscopy

  • Sterilization and disinfection techniques

Unit II: Microbial Physiology and Growth

  • Microbial nutrition and growth requirements

  • Growth curve and measurement techniques

  • Culture media and methods

  • Physical and chemical control of microorganisms

Unit III: Microbial Genetics

  • DNA replication, transcription, translation in prokaryotes

  • Genetic recombination: Transformation, Transduction, Conjugation

  • Plasmids and transposons

  • Mutation and repair mechanisms

Unit IV: Introduction to Immunology

  • Innate and adaptive immunity

  • Cells and organs of the immune system

  • Antigens and antibodies: Structure and function

  • Antigen-antibody interactions

Unit V: Immune Mechanisms and Applications

  • Major Histocompatibility Complex (MHC)

  • Cytokines, Complement system

  • Hypersensitivity reactions

  • Immunological techniques: ELISA, Western blotting, Immunofluorescence

  • Vaccines and immunization

Course Outcomes (COs):

By the end of the course, students will be able to:

  1. Understand microbial diversity, structure, and function.

  2. Apply knowledge of microbial growth and control techniques.

  3. Explain molecular mechanisms of microbial genetics.

  4. Describe immune system components and their functions.

  5. Interpret immunological responses and diagnostic applications.

Textbooks and References:

  • Prescott’s Microbiology – Willey, Sherwood, and Woolverton

  • Microbiology – Pelczar, Chan, and Krieg

  • Kuby Immunology – Kindt, Goldsby, Osborne

  • Janeway’s Immunobiology – Murphy and Weaver